AProposalforResearch on Spinal Cord RepairUsing Specific

Frequenciesof Sound/Ultrasound

By PhysicistGary Wade

 

 

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†††† There is reason to believe that by the use of specific frequencies of mechanical vibration (sound/ultrasound) that scar tissue generated from spinal cord injury can be made to go embryonic-looking.Furthermore, that once these scar tissue cells have become embryonic-like or looking (dedifferentiation) they can then redifferentiate into the normal glia and other type cells needed to repair the spinal cord.

 

†††† The belief in the possibility of converting scar tissue into embryonic like cells that will redifferentiate into the needed normal cells for repair is based on several sources: 1)the work of Robert Becker, MD, 2)the physical structures of ion gates or ion channels on the various human cell types, and 3)my own work of converting fibroblast cells in scar tissue into the type of cell(s) that should be there, if the scar tissue were not there.

 

†††† Dr. Robert O. Becker, MD, demonstrated experimentally that both the salamander and mammals have the same tissue regeneration mechanisms.These regeneration mechanisms come into play after severe traumatic tissueinjury (ref. 1).However, the mammal in general compared to the salamander is deficient in the ability to send a sufficient negative electric current to the damaged region, which manifests itself as hydroxylions generation in the damaged tissue region (ref. 2).Becker demonstrated that if the mammalís ability to supply a negative electric current to a severely damaged region ( i.e. amputated leg) was artificially supplemented to the level comparable to that used by a salamander, then the mammal (rat) would re-grow the amputated leg (ref. 3).One of the necessities for mammals to regenerate damaged tissue is for some cell types to dedifferentiate into a embryonic-like cells and then redifferentiate into the needed cell types for tissue repair or regeneration.In salamanders, their red blood cells, which unlike those in mammals are nucleated, play this role.In mammals Becker demonstrated that fibroblast cells could be made to look embryonic and presumably can carry out this same role in mammals.

 

†††† In experiments carried out at the Center for Complex Infectious Diseases (CCID) in Rosemead, CA, I was able to get fibroblast cells and kidney epithelial cells to go embryonic-like or looking using a type of pulsed magnetic field configuration.Apparently the type of pulsed magnetic field configuration used has the ability to open various cell membrane ion gates and then force feed them with their own ion type.(For more information on this pulsed magnetic field work check my web site, rifeenergymedicine.com, for my soon to be web published article on this and other energy medicine work).It is apparently the appropriate changing of ion concentrations in the fibroblast and other cell type cytoplasm and therefore cell nucleus region which causes cell dedifferentiation to an embryonic like state.It is known experimentally that metal ion water complexes can interact with chromosome DNA to open and close gene sequences.For example, conversion of Z-DNA to B-DNA by changing positive metal ion water complex concentrations (Ref. 4).

 

†††† In field trials carried out with the same type of magnetic pulse equipment used at CCID, we have been able to repair all manor and kind of fibroblast cell based scar tissue and would be scar tissue damage in horses and humans.However, we have been unsuccessful with this equipment use on spinal cord scar tissue, which is not fibroblast cell based.This does not mean that there might not be some other pulsed magnetic field approach that would work to convert spinal cord scar tissue to the needed normal spinal cord tissue structure.

 

†††† Since, as Dr. Becker has shown, the principles and mechanism of body repair and tissue regeneration for the salamander and mammals seems to be essentiallyidentical, should we not expect a mammal to be able to repair or regenerate from spinal cord injury, the same way a salamander does, if we just artificially facilitate repair conditions?One way to artificially facilitate repair conditions is to open up specific ion gates on spinal cord scar tissue cells and change their cytoplasm ion concentrations and have them become embryonic-like.Following from Beckerís work and my own work with pulsed magnetic fields, it should be expected that the scar tissue cells at the surface of the scar tissue, after they convert to embryonic like cells, will redifferentiate to the type of cell that should be there, if no scar tissue were there at the cellsí location.In studying fibroblast cell cultures exposed to pulsed magnetic fields, it was only the fibroblast cells at the edge of the cell culture that converted over to embryonic-looking cells.The fibroblast cells in the bulk of these cell cultures were butted up against each other and presumably physically connected together.When these cells were exposed to the magnetic pulses they showed some subtle transient morphological changes but continued to look like fibroblast cells.†† It was therefore found as expected that several, to many repeated exposures of the scar tissue, in field trials, were required to entirely convert the scar tissue to normal tissue.The scar tissue is a matrix of mainly fibroblast cells and collagen fibers.The fibroblast cells generate and maintain the triple stranded electrically conductive protein collagen, which gives the scar tissue its connective strength.As the fibroblast cells are converted to normal tissue cells, they no longer maintain the collagen matrix and in time it goes away leaving normal tissue behind.

 

†††† Through the repeated (once per 24 hours) short exposure times (perhaps 5 to 15 minutes) of spinal cord scar tissue cells to specific frequencies of sound/ultrasound that open up specific ion gates, the scar tissue surface cells can possibly be made to go embryonic-like and then turn into normal healthy neural tissue and therefore spinal cord repair is accomplished.With this possibility at hand it becomes a question of what are the various mechanical vibration frequencies for the various metal ion gates and other ion gates used by scar tissue cells.

 

†††† When looking in the literature at the various now-known ion gate structures, a familiar pattern appears.That pattern is that in general the ion gates are made up of several nearly identical elongated trans bi-lippid membrane proteins (see Figures 1A, Figure 1B, and Figure 1C).†† Looking in a direction at right angles to the cell membrane surface into the cell these membrane crossing proteins form a circular closed-on-themselves pattern.In the middle of this circular protein pattern, about half way through the cell bi-lipid membrane, the protein molecules are very close together and they have amino acid sequences that only allow the ion water complex for which the gate is for to be there.When the appropriate signal is received by the ion gate, the center channel slightly enlarges or is unplugged and that ion type streams into or out of the cell depending on which ion gate is activated.Because these ion gate structures in general, from a physics point of view, are essentially periodically spaced masses which areelastically coupled together and form a closed back-on-itself system, they will have specific mechanical vibration frequencies with which they will go into resonant vibration.This is a known fact from the old German mathematician eigenfunction problem/solution for the standing waveson astring with periodically spaced mass beads of equal mass and with circular boundary conditions (closed back on itself).What this means is, that if these ion gate structures are exposed to their primary natural mechanical resonance frequency, they can become open to ion transport for a significant part of each resonant cycle.This is because the opening up and closing down of the central ion channel cross sectional area is part of the resonant mechanical vibration motion.In other words, the gates become very leaky and are effectively open while exposed to their primary resonance frequency.Because of the relatively large masses of the ion gate proteins and the relatively low strength of elastic coupling between gate proteins, the gate resonance frequencies should be relatively low;Perhaps as low as several hundred to several thousand cycles per second.

 

The Needed Experiments

 

†††† At some university or other research facility that has in operation labs that use ion type specific microscopic probes for monitoring specific ion typetransport across cell membranes, a set of experiments needs to be performed;†† Namely, monitoring a specific cellís ion transport of various ion types across the cell membrane as a function of exposure tomechanical vibration frequency.In our particular case, we are interested in studying the spinal cord scar tissue cells of both rats and humans.Once the various gate frequencies for the various metal and other ions are obtained, experiments can be performed to find the frequency combinations, time of exposures, and intensities to make the scar tissue cells in rats and humans go embryonic- like, but without cell deathand/or cell damage.From here, a short period of tests on rats with induced spinal cord scar tissue can be carried out. If the tests with rats are successful, then on to human trials.†† If the FDA is being consulted and puts up its normal ďlet us wait for a few more years to test on humansĒ routine, immediately do the human trial out of the country.

 

Note:There is no legitimate reason to put other species of test animals in the hundreds to thousands, into misery doing these spinal cord tests.Any research institution that thinks mass animal tests are required, should not be involved in this research.

 

 

References:

1)     The Body Electric, Electromagnetism and the Foundation of Life, by Robert O. Becker, M.D., and Gary Selden, and illustrated by David Bichell;ISBN 0-688-06971-1

2)     A Physicistís View of the Use of Feeble Electric Direct Currents to Repair Tissue and Replace Body Parts (Part One), by Gary Wade, Health Freedom News, February 1996, Pages 22 to 33. (see attached article).

3)     The Body Electric, Pages 152 to 155.

4)     The DNA Helix and How It Is Read, Scientific American, December 1983, Vol. 249, No, 6, pp. 94-111.

 

 

 

Note to would-be researchers:

 

†††† In my experiments with sound/ultrasound generation for experimentation on microbes and cell cultures, I have used 1/8th pie cut pieces of the standard 2 inch in diameter and 1/10 inch thick circular piezoelectric transducer elements that are used in ultrasonic cleaner tanks.These 1/8th pie cut pieces are easily ran with the standard off the shelf electronic tech signal function generator found In physics labs and used by electronic techs.I usually epoxy the 1/8th cut element to a small metal rectangular piece and then clamp or fixture the metal plate to the microscope stage or slide holder or side of dish.The standard piezoelectric elements are polarized such that one side of the transducer element should always be maintained at a positive potential to the other side, so that the element does not degrade in its ability to generate mechanical vibrations with applied electrical voltage oscillations.If higher amplitude of mechanical vibration is desired or required, the signal from the signal function generator can be amplified by using a linear amplifier.For example, if the frequency range of interest is from say 20 cycles per sound (cps) to 30,000 cps then a good audio amplifier will do the job.†† This frequency range is expected to be the range in which the various ion gates on cell membranes will have their resonance frequencies.The piezoelectric element only transforms voltage sign waves into pressure sine waves.

†††† Keep in mind that one side of the piezoelectric element needs to be free to expand and contract in the air or liquid environment being used for experimentation.

†††† For the frequency range above, standard off the shelf speakers can be used.However, some kind of sound enclosure will be needed so as not to bug everyone to tears.


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